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Clinical Chemistry 21: 1892-1898, 1975;
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Clinical Chemistry, Vol 21, 1892-1898, Copyright © 1975 by the American Association for Clinical Chemistry

Gas-Chromatographic/Mass-Spectrometric Identification and Quantitation of Tetronic and Deoxytetronic Acids in Urine from Normal Adults and Neonates

John A. Thompson 1, Sanford P. Markey 1, and Paul V. Fennessey 1

1 B. F. Stolinsky Research Laboratories, Department of Pediatrics, University of Colorado Medical Center, Denver, Colo. 80220.

Gas chromatographic/mass spectrometric/computer analyses of trimethylsilyl derivatives of urine extracts, prepared by an anion-exchange procedure, reveal the presence of several tetronic and deoxytetronic acids. Identifications were confirmed by comparing gas-chromatographic methylene unit values and mass spectrometric characteristics with those of chemically synthesized samples. In addition, several 1,4-lactones corresponding to the acids were detected in small amounts. The acids were measured in groups of adults and neonates by gas chromatographic/computer determination of peak areas and the use of calculated response factors. In urines from adults, 4-deoxythreonic, threonic, and erythronic acids were present in relatively large amounts. 4-Deoxyerythronic and 2-deoxytetronic acids were present in small to moderate amounts; 3-deoxytetronic and 2-methylglyceric acids were only trace constituents. These latter two acids, in addition to 4-deoxyerythronic, were also trace constituents of several urines from neonates but could not be detected in others. Expressed in terms of creatinine excretion, both groups excreted similar amounts of 4-deoxythreonic acid and neonates excreted significantly more 2-deoxytetronic, erythronic, and threonic acids.

Submitted on May 19, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.