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Clinical Chemistry 21: 199-205, 1975;
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Clinical Chemistry, Vol 21, 199-205, Copyright © 1975 by the American Association for Clinical Chemistry

Kinetic Equations and Error Analysis for the Cholesterol—Sulfuric Acid Reaction

Thomas E. Hewitt 1 and Harry L. Pardue 1

1 Department of Chemistry, Purdue University, Lafayette, Ind. 47907.

Quantitative data reported in an earlier paper have been used to evaluate kinetic equations for the reaction of cholesterol with sulfuric acid in acetic acid—acetic anhydride medium. The equations satisfy experimental observations to within 6% over a wide range of experimental conditions. The equations have been used to justify mathematically the empirically developed measurement procedures described earlier and to predict the errors that can be expected for time and temperature uncertainties.

Submitted on October 5, 1974
Accepted on October 24, 1974







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Copyright © 1975 by the American Association for Clinical Chemistry.