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Clinical Chemistry 21: 216-220, 1975;
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Clinical Chemistry, Vol 21, 216-220, Copyright © 1975 by the American Association for Clinical Chemistry

Determination of Serum Thyroxine after Dissociation from Thyroxine-Binding Globulin in Alkaline Solution and Adsorption on Dextran-Coated Charcoal

William H. Wong 1

1 Department of Chemistry, CLMG, Inc., 679 S. Westlake Ave., Los Angeles, Calif. 90057.

A competitive protein-binding procedure for determining serum thyroxine is described in which thyroxine is first dissociated from serum thyroxine-binding globulins in an alkaline solution. After binding equilibration, the unbound thyroxine is separated by dextran-coated charcoal. The procedure takes only 100 µl of serum, requires no evaporation, and is sensitive and reproducible. Results by this procedure correlate well with those for a generally accepted method.

Submitted on August 5, 1974
Accepted on November 6, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.