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Clinical Chemistry 21: 424-426, 1975;
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Clinical Chemistry, Vol 21, 424-426, Copyright © 1975 by the American Association for Clinical Chemistry

Lipase-Triggered Kinetic Assay of Serum Triglycerides

Giovanni Bucolo 1, Ralph McCroskey 1, and Nadine Whittaker 1

1 Biochemical Research Laboratories, Calbiochem, 10933 North Torrey Pines Rd., La Jolla, Calif. 92037.

We describe a kinetic method for assay of serum or plasma triglycerides, by use of an enzymatic hydrolysis and reaction sequence already described [Clin. Chem. 19, 476 (1973)]. The reaction is triggered by addition of lipase, at a time when free glycerol, or pyruvate (or both) are no longer present. In this method, therefore, there is no need for a blank glycerol assay. In the procedure, reagents are used that are available commercially in the form of stable, dry powders; the method for the preparation of the reagents has been changed to achieve improved stability and performance. Stability and recovery of added triglycerides are satisfactory.

Submitted on June 25, 1974
Accepted on December 24, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.