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Clinical Chemistry 21: 428-431, 1975;
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Clinical Chemistry, Vol 21, 428-431, Copyright © 1975 by the American Association for Clinical Chemistry

Simultaneous Determination of Serum Cholesterol and Triglycerides after Preliminary Column Chromatography

Godfrey Moses 1, Edwin Olivero 1, and Thomas F. Draisey 1

1 The Salvation Army Grace Hospital, Windsor, Ontario N9A 5C6 (Canada)

An isopropanolic extract of serum can be made suitable for the simultaneous estimation of cholesterol and triglycerides by passing it through a commercially-available chromatographic column containing activated metallic oxides in which alumina predominates. No centrifugation step nor phase separation is required. Use of the purified extract allows existing methods to be simplified and shortened without loss of reproducibility or stability. Results compare well with those obtained by traditional methods.


Key Words: chromatography on activated metallic oxides

Submitted on April 15, 1974
Accepted on January 3, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.