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Clinical Chemistry 21: 588-590, 1975;
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Clinical Chemistry, Vol 21, 588-590, Copyright © 1975 by the American Association for Clinical Chemistry

Effect of the Matrix of the Standard on Results of Atomic Absorption Spectrophotometry of Zinc in Serum

Berlslav Momccaronilovicacute 1, Bartholomeus Belonje 1, and Bhagwan G. Shah 1

1 Bureau of Nutritional Sciences, Health Protection Branch, Tunney's Pasture, Ottawa, Ontario, Canada, K1A OL2.

Zinc in pooled human serum, diluted 10-fold with demineralized water, was determined by flame atomic absorption spectrophotometry. Recoveries of zinc added to the serum were calculated by use of standards prepared in water, physiological saline, synthetic serum, or dilute HNO3 (10 ml/liter). The flow rates were essentially the same (9.1 to 9.3 ml/min) for water, diluted synthetic serum or actual human serum, and diluted nitric acid. The mean apparent percentage recoveries (±SD) were the best (102.6 ± 2.5) when the standards were prepared in nitric acid; the values obtained when standards in water were used were too high (111.5 ± 3.9); those with standards prepared in physiological saline (92.0 ± 3.2) or synthetic serum (92.3 ± 2.5) were too low.

Submitted on November 14, 1974
Accepted on January 29, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.