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Clinical Chemistry 21: 619-625, 1975;
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Clinical Chemistry, Vol 21, 619-625, Copyright © 1975 by the American Association for Clinical Chemistry

Determination of Serum Copper by Atomic Absorption, with Use of the Graphite Cuvette

Merle A. Evenson 1 and Brenda L. Warren 1

1 Department of Medicine and Clinical Toxicology Laboratories, University of Wisconsin Hospitals, Madison, Wis. 53706.

We have established and evaluated a flameless graphite cuvette method for copper in serum. This atomic absorption method provides substantial improvement in sensitivity, adequate accuracy, and acceptable precision, and little sample preparation is required before the analysis. Standard addition studies and measurements of National Bureau of Standards materials indicated that the proposed method is accurate, but that sample pH must be kept between 2 and 3 for high accuracy. Cations and anions that frequently are present in proteincontaining samples do not interfere significantly. Sample cross contamination in the syringe must be carefully avoided. Finally, when results for more than 100 patients' sera by this method were compared to those obtained by flame atomic absorption for the same samples, no substantial bias or inaccuracies could be attributed to this new micro-scale method for serum copper. Hence, this method is ideally suited for use on pediatric patients.


Key Words: trace elements • pediatric chemistry • flame atomic absorption method compared • diagnostic aid • use of aqueous standard

Submitted on December 30, 1974
Accepted on January 31, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.