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Clinical Chemistry 21: 685-693, 1975;
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Clinical Chemistry, Vol 21, 685-693, Copyright © 1975 by the American Association for Clinical Chemistry

Use of Carbon Dioxide- and Oxygen-Tonometered Phosphate— Bicarbonate—Chloride—Glycerol—Water Mixtures for Calibration and Control of pH, pco2 and pO2 Electrode Systems

A. H. Veefkind 1, R. A. M. Van den Camp 1, and A. H. J. Maas 1

1 Department of Medical Physics, Free University, Amsterdam, and Department of Cardiology and Cardiovascular Surgery, University Hospital, Utrecht, The Netherlands.

Calibration of pH, pco2, and po2 electrode systems of modern blood—gas analyzers, designed with one sample cuvet for measurement, is mostly performed separately with buffer solutions and gas mixtures. We describe the development of solutions of known pH, pco2 and po2 for doing such calibrations simultaneously, containing phosphate, bicarbonate, and chloride in glycerol—water mixtures as solvent. A method is suggested for computing the relation between pH and log pCO2 of these solutions in equilibrium with carbon dioxide gas. It is demonstrated that a solution of phosphate (Na2HPO4, KH2PO4, each 25.mmol/liter), bicarbonate (NaHCO3, 30 mmol/ liter), and chloride (NaCI, 30 mmol/ liter) in glycerol-water mixture (3/7 by vol) and equilibrated with CO2 in air (4 vol/100 vol) and CO2 in nitrogen (8 vol/100 vol), respectively, makes possible accurate and simultaneous calibration of the pH, pco2, po2 electrodes of a Corning Model 165 blood—gas analyzer. Similar solutions may also be used for quality—control of blood—gas measurement.


Key Words: blood gases • acid—base balance • calibration of blood—gas analyzers, pH meters • quality—control • partial pressures of gases

Submitted on December 3, 1974
Accepted on January 22, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.