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Clinical Chemistry 21: 703-707, 1975;
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Clinical Chemistry, Vol 21, 703-707, Copyright © 1975 by the American Association for Clinical Chemistry

Direct Determination of Total Serum Cholesterol by Use of Double-Wavelength Spectrophotometry

Philip B. Sommers 1, Peter I. Jatlow 1, and David Seligson 1

1 Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Conn. 06510.

We describe a simple, accurate method for direct determination of total cholesterol in serum. Systematic investigation of a previously described modified Liebermann— Burchard reagent has indicated the necessity of accounting for both bilirubin interference and decreased specificity owing to exothermia. Double-wavelength spectrophotometry was used to optically null out bilirubin as an interfering factor, whereas adding serum to the cold reagent increases its specificity for the cholesterol color reaction. Comparison of 106 cholesterol values with those obtained by the procedure of Abell et al. [J. Biol. Chem. 195, 357 (1952)] yielded a correlation coefficient greater than 0.99; our inter-run coefficient of variation for pooled laboratory serum was 1.7%.


Key Words: comparison with Abell method • billirubin interference • reagent specificity

Submitted on January 17, 1975
Accepted on January 30, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.