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Clinical Chemistry 21: 741-745, 1975;
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Clinical Chemistry, Vol 21, 741-745, Copyright © 1975 by the American Association for Clinical Chemistry

Thermochemical Determination of Glucose in Serum, Plasma, and Whole Blood without Prior Deproteinization

C. David McGlothlin 1 and Joseph Jordan 1

1 Department of Chemistry, The Pennsylvania State University, 152 Davey Laboratory, University Park, Pa. 16802.

Glucose can be determined by phosphorylatlon in the presence of hexokinase (EC 2.7.1.1) by using Mg2+/ (ATP)2- as the phosphorylating agent. A novel instrumental quantitation method is described, direct injection enthalpimetry, whereby the heat of the enzymatically catalyzed reaction was measured. The reaction was allowed to proceed to virtual completion (99.5+%) in an adiabatic Dewar vessel at 25.00 ± 0.01 °C (range). Samples were sequentially injected into a reaction mixture consisting of hexokinase, ATP, Mg2+, and a tris(hydroxymethyl)aminomethane buffer (pH 8). Single analyses required <2 min for 0-3 g/liter samples. The linear dynamic range was 0.3-10 g/liter, with zero intercept and a precision and accuracy of 2%. Electrical calibration in situ obviated the need for chemical standards, and because protein and color do not interfere, no sample pretreatment was necessary before analysis.


Key Words: physical chemical approach to glucose measurement • enzymatic assay • analytical system • thermochemistry • emergency determinators

Submitted on December 23, 1974
Accepted on March 3, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.