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Clinical Chemistry 21: 864-872, 1975;
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Clinical Chemistry, Vol 21, 864-872, Copyright © 1975 by the American Association for Clinical Chemistry

Calibration of Ion-Selective Electrodes for Use in Biological Fluids

M. S. Mohan 1 and Roger G. Bates 1

1 Department of Chemistry, University of Florida, Gainesville, Fla. 32611.

We measured electromotive force at 37 °C with pH, sodium glass, valinomycin, and the Simon calcium electrodes in synthetic electrolyte mixtures simulating serum, in cells both with and without liquid junction. Evidently these electrodes respond in a near-Nernstian manner to changes in concentration of the ion sensed. We suggest that such mixtures may serve as calibrating standards for ion-selective electrodes in clinical analysis. The effect of ionic strength on electrode response can be accounted for almost wholly by alterations in the activity coefficients. The lanthanum fluoride electrode shows promise as a reliable reference in cells without liquid junction.


Key Words: electrolytes • liquid junction potential • lanthanum fluoride electrode

Submitted on March 12, 1975
Accepted on March 24, 1975




The following articles in journals at HighWire Press have cited this article:


Home page
 Hum Exp ToxicolHome page
A.B.T.J. Boink, J. Wemer, J. Meulenbelt, H.A.M.G. Vaessen, and D.J. de Wildt
The Mechanism of Fluoride-Induced Hypocalcaemia
Human and Experimental Toxicology, January 1, 1994; 13(3): 149 - 155.
[Abstract] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.