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Clinical Chemistry 21: 880-883, 1975;
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Clinical Chemistry, Vol 21, 880-883, Copyright © 1975 by the American Association for Clinical Chemistry

Enzyme Activities of NADPH-Forming Metabolic Pathways in Normal and Leukemic Leukocytes

Francesco Belfiore 1, Vito Borzi 1, Luigi Lo Vecchio 1, Elena Napoli 1, and Agata M. Rabuazzo 1

1 Clinica Medica dell’Università di Catania, Ospedale Garibaldi, 95123 Catania, Italy.

With respect to the enzymes of NADPH-forming metabolic pathways in human leukocytes: (a) Glucose-6phosphate dehydrogenase and phosphogluconate dehydrogenase (decarboxylating) were less active in leukocytes (mostly myeloblasts) from eight patients with acute myeloblastic leukemia (I) than in leukocytes (mostly granulocytes) from 16 normal subjects (II) or 16 patients with chronic myelocytic leukemia (III). (b) Of the enzymes of the citrate cleavage pathway, ATP citrate lyase and malate dehydrogenase (decarboxylating) (NADP+) were virtually absent in the cells studied. (c) Isocitrate dehydrogenase (NADP+), aspartate aminotransferase, and alanine aminotransferase, which, together with the much more active malate dehydrogenase, constitute a newly proposed NADPH-forming metabolic cycle, showed a higher activity in I than in II or III, and therefore could compensate, as concerns NADPHgeneration, for the low activity of pentose cycle dehydrogenases. We are not sure whether the enzymatic characteristic of I cells is attributable to their immaturity or to their leukemic nature.


Key Words: pentose phosphate pathway NADPH-forming cycle

Submitted on January 31, 1975
Accepted on March 28, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.