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Clinical Chemistry 21: 1136-1140, 1975;
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Clinical Chemistry, Vol 21, 1136-1140, Copyright © 1975 by the American Association for Clinical Chemistry

New Colorimetric Reaction for End-Point, Continuous-Flow, and Kinetic Measurement of Urea

David Jung 1, Homer Biggs 1, John Erikson 1, and Peggy U. Ledyard 1

1 Indiana University School of Medicine, Indianapolis, Ind. 46202.

A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 µl of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good sensitivity. A major advantage of the reaction is that it occurs at a temperature of 37 °C or lower. The results obtained by all three methods agree well with those for a continuous-flow procedure in which diacetyl is a reagent.


Key Words: intermethod comparison

Submitted on December 2, 1974
Accepted on May 5, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.