Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 1169-1171, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lenz, P. H.
Right arrow Articles by Toth, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lenz, P. H.
Right arrow Articles by Toth, J. M.

Clinical Chemistry, Vol 21, 1169-1171, Copyright © 1975 by the American Association for Clinical Chemistry

Standardization of Incubation Time and Temperature for Eight Radioimmunoassays

Paul H. Lenz 1, George H. Wien 2, and Janice M. Toth 2

1 Department of Biological Sciences, Fairleigh Dickinson University, Madison, N. J. 07940.
2 Wien Laboratories, Inc., P. O. Box 227, Succasunna, N. J. 07876.

We wanted to know if incubation time and temperature for many radioimmunoassay methods could be standardized, to decrease assay time and so improve efficiency. Percentage binding was determined for triiodothyronine, thyroxine, digoxin, digitoxin, testosterone, aldosterone, estradiol, and diphenylhydantoin when the reaction mixtures were incubated at 6-8, 22, or 37 °C for various periods of time. In all methods tested, binding of antigen to antibody was greatest when the reaction mixtures were incubated at 6-8 °C, less at 22 °C, and least at 37 °C. Maximum binding occurred within 30-60 min at each temperature for all methods tested. Hence, it is possible to standardize the incubation time and temperature for these eight radioimmunoassay methods to 60 min at 6-8 °C.


Key Words: "kit" methods • hormones • toxicology • analytical error

Submitted on February 10, 1975
Accepted on April 10, 1975






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.