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Clinical Chemistry 21: 1294-1296, 1975;
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Clinical Chemistry, Vol 21, 1294-1296, Copyright © 1975 by the American Association for Clinical Chemistry

Gas-Chromatographic and Mass-Spectrometric Detection of Low-Molecular-Weight Aliphatic Alcohols in Urine of Normal Individuals and Patients with Diabetes Mellitus

Hartmut M. Liebich 1, Ossama Al-Babbili 1, Albert Zlatkis 2, and Kyoung Kim 2

1 Medizinische Universitätsklinik, Abteilung IV, Otfried-Mueller-Strasse, 74 Tuebingen, Germany.
2 Chemistry Department, University of Houston, Houston, Tex. 77004.

We studied the aliphatic alcohols in 100 urines from 25 patients with diabetes mellitus under treatment with insulin, oral antidiabetic medication, or special diet. The procedure involves adsorption of the low-molecular-weight urinary metabolites on a porous polymer of 2,6-diphenyl-p-phenylene oxide (Tenax GC), gas-chromatographic separation, mass spectrometric identification, and mass fragmentographic representation of the primary alcohols by a computer. The concentrations of ethanol, n-propanol, isobutanol, n-butanol, and isopentanol are increased as compared with urine from normal persons.

Submitted on May 11, 1975
Accepted on May 30, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.