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Clinical Chemistry 22: 1306-1309, 1976;
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Clinical Chemistry, Vol 22, 1306-1309, Copyright © 1976 by American Association for Clinical Chemistry

Purification of alpha1-fetoprotein

SL Twomey and RV Sweet

Previously published methods for purifying alpha1-fetoprotein are inadequate because they either do not yield a completely pure product or they cause some denaturation. We present a method that does not have these serious disadvantages, and with which alpha1-fetoprotein was purified by sequential use of concanvalin A affinity-chromatography, preparative gel-electrophoresis, and immunoabsorption with anti-albumin antibody covalently coupled to Sepharose 4B. The purity of the product was monitored by discontinuous polyacrylamide-gel electrophoresis and counterimmunoelectrophoresis, both of which must be used to ascertain what proteins are present at each step of the purification.




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Copyright © 1976 by the American Association for Clinical Chemistry.