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Clinical Chemistry 23: 86-88, 1977;
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Clinical Chemistry, Vol 23, 86-88, Copyright © 1977 by American Association for Clinical Chemistry

Combined gel filtration, biuret/copper method compared with an immunochemical method for urinary protein measurement

JD Peele Jr, RH Gadsden and CB Loadholt

We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were analyzed by each method. There was linear agreement and a correlation of 0.96 between the two methods, but urinary protein values determined by the immunochemical method, especially for patients with multiple myeloma, were lower than by the chemical method.




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Copyright © 1977 by the American Association for Clinical Chemistry.