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Clinical Chemistry 23: 1868-1872, 1977;
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Clinical Chemistry, Vol 23, 1868-1872, Copyright © 1977 by American Association for Clinical Chemistry

Radioimmunoassay for digoxin with a fully automated continuous-flow system

H Greenwood, J Landon and GC Forrest

We describe a fully automated continuous-flow radioimmunoassay for digoxin with use of a Technicon AutoAnalyzer system. It makes use of antibodies linked to magnetizable particles; a magnet separates the bound and free fractions. The precise dispensing of reagents by the AutoAnalyzer system permits a small incubation volume (about 160 microliter) and the reproducible timing enables non-equilibrium conditions to be used. Thus a single sample is assayed in 15 min, with a sample throughput of 30/h. The standard curve ranges from 0.5 to 8.0 microgram/liter and is most precise between 1 and 3 microgram/liter, the between-assay coefficient of variation being less than 3%. There is no significant carryover between samples of high and low concentration, and results by the method correlate closely (r = 0.969) with those by an established manual assay in which charcoal separation is used.


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 Clin. Chem.Home page
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The Evolution of Immunoassay as Seen Through the Journal Clinical Chemistry
Clin. Chem., October 1, 1998; 44(10): 2071 - 2074.
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Copyright © 1977 by the American Association for Clinical Chemistry.