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Clinical Chemistry 23: 2151-2154, 1977;
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Clinical Chemistry, Vol 23, 2151-2154, Copyright © 1977 by American Association for Clinical Chemistry

Determination of urinary total phenolic compounds with use of 4- aminoantipyrine: suggested screening test for hyperthyroidism and for catecholamine-producing tumor

Y Yamaguchi and C Hayashi

We describe a method for determining those urinary total phenolic compounds that are tyrosine analogs or metabolites, such as thyroxine and catecholamines. The urine sample, 4-aminoantipyrine in carbonate- bicarbonate buffer, and potassium ferricyanide solution are mixed and the quinoneimine dye that forms is measured at 500 nm. Some cases of hyperthyroidism, diabetes mellitus, nephrosis, obesity, hypertension, or catecholamine-producing tumor showed above-normal values, so that this determination seems useful as a screening test for these disorders.


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