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Clinical Chemistry 23: 169-174, 1977;
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Clinical Chemistry, Vol 23, 169-174, Copyright © 1977 by American Association for Clinical Chemistry

Radioimmunoassay of lysergic acid diethylamide (LSD) in serum and urine by using antisera of different specificities

WA Ratcliffe, SM Fletcher, AC Moffat, JG Ratcliffe, WA Harland and TE Levitt

We raised high-titre antisera to two LSD-bovine serum albumin conjugates, one linked via the indole nitrogen, the other via the amide side-chain. The antisera were specific for different parts of the LSD molecule, as demonstrated by cross-reactivity studies with LSD, its metabolites, ergot alkoloids, and closely related compounds. The antisera were used to develop a double-antibody radioimmunoassay with a detection limit of about 0.4 mug of LSD per liter of unextracted urine or serum. We saw no nonspecific interference by urine, serum, or from a series of commonly used drugs. There was good correlation between immunoassay values obtained with the two antisera (r = 0.91). However, the antiserum linked via the indole nitrogen gave consistently higher results for samples from persons who had taken LSD, owing to greater cross-reactivity with LSD metabolites. Radioimmunoassay by use of two such antisera is a more specific screening procedure for LSD abuse than has been available previously. In addition, antisera cross-reacting with LSD metabolites allow measurement of these compounds, for which there is no satisfactory method at the concentrations found in biological fluids in man.


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S. Kerrigan and Donald. E. Brooks
Indirect enzyme-linked immunosorbent assay for the quantitative estimation of lysergic acid diethylamide in urine
Clin. Chem., May 1, 1998; 44(5): 985 - 990.
[Abstract] [Full Text] [PDF]


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