Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 23: 223-228, 1977;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wakayama, J. E.
Right arrow Articles by Swanson, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wakayama, J. E.
Right arrow Articles by Swanson, J. R.

Clinical Chemistry, Vol 23, 223-228, Copyright © 1977 by American Association for Clinical Chemistry

Ultraviolet spectrometry of serum triglycerides by a totally enzymic method adapted to a centrifugal analyzer

JE Wakayama and JR Swanson

We described a micromethod for determining serum triglycerides (triacylglycerols) with the centrifugal analyzer. This technique is based on the procedure of Bublitz and Kennedy [J. Biol. Chem. 211, 951 (1954)]. The enzymic hydrolysis requires 10 min at 30 degrees C. Twenty- six serum triglyceride assays can be done in about 30 min. Concentration and absorbance are linearly related up to 5.0 mmol/liter, but higher concentrations can be assayed by changing conditions slightly. Day-to-day reproducibility for the method was satisfactory (CV, 2.7 to 8.4%). Comparison of this method and two other methods for triglycerides, the automated Hantzsch condensation method and a commercial enzymic method (Calbiochem), gave correlation coefficients of 0.976 and 0.990, respectively. Results are unaffected by the presence of endogenous serum ADP, pyruvic acid, phosphatases, or ATPase.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1977 by the American Association for Clinical Chemistry.