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Clinical Chemistry 23: 518-521, 1977;
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Clinical Chemistry, Vol 23, 518-521, Copyright © 1977 by American Association for Clinical Chemistry

Cortisol production rate determined by radio gas-chromatography

HJ Derks, FA Muskiet, BG Wolthers, JH Thijssen and NM Drayer

We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducible, the coefficients of variation ranging from 8-15% of allotetrahydrocortisol/tetrahydrocortisol and from 9-19% for tetrahydrocortisone. Correlation coefficients were 0.966 and 0.998 for tetrahydrocortisone and allotetrahydrocortisol/tetrahydrocortisol, respectively, when the method was compared with a method involving thin- layer chromatography and colorimetry. Only one chromatographic step is needed for both purification and quantitation, thus time and effort are saved.




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Copyright © 1977 by the American Association for Clinical Chemistry.