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Clinical Chemistry 23: 879-881, 1977;
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Clinical Chemistry, Vol 23, 879-881, Copyright © 1977 by American Association for Clinical Chemistry

New substrate for fluorometric determination of gamma- glutamyltransferase activity in serum

JD MacQueen, RC Driscoll and RJ Gargiulo

We describe the preparation of a new substrate, N-gamma-L-glutamyl-5- aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).




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Copyright © 1977 by the American Association for Clinical Chemistry.