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Clinical Chemistry 23: 994-999, 1977;
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Clinical Chemistry, Vol 23, 994-999, Copyright © 1977 by American Association for Clinical Chemistry

Effect of analytical factors on immunochemical reference limits for complement component C3 in serum of a reference pediatric population

GJ Buffone and SA Lewis

We evaluated analytical factors such as antibody specificity, standard materials, and methodology for the measurement of C3. Mancini-type radial immunodiffusion and immunonephelometry were shown to give comparable data if variables other than procedural variables are eliminated. The most significant analytical factors affecting the measurement were antiserum specificity and source of standard material.





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Copyright © 1977 by the American Association for Clinical Chemistry.