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Clinical Chemistry 23: 1258-1263, 1977;
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Clinical Chemistry, Vol 23, 1258-1263, Copyright © 1977 by American Association for Clinical Chemistry

Continuous-flow determination of dialyzable calcium in serum

J Toffaletti, J Savory and HJ Gitelman

We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is fluorometrically measured. Analysis of samples containing protein, prepared by an equilibrium dialysis technique, shows that protein-bound calcium is not detected. The method requires less than 0.4 ml of serum, and is accurate, precise, and durable. No unusual care or special techniques are required during sample collection or analysis.




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Copyright © 1977 by the American Association for Clinical Chemistry.