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Clinical Chemistry 23: 1386-1388, 1977;
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Clinical Chemistry, Vol 23, 1386-1388, Copyright © 1977 by American Association for Clinical Chemistry

Preparation of a phospholipase C-antihuman IgG conjugate, and inhibition of its enzymatic activity by human IgG

R Wei and S Riebe

We labeled IgG with phospholipase C, using 1-cyclohexyl-3-(2- morpholinoethyl)-carbodiimide. Enzymaticactivity of the resulting conjugate was inhibited when it was complexed with human IgG, but rabbit or goat IgG was not effective in suppressing the enzyme activity. Normal erythrocytes were used as substrate for the enzyme, enzymatic activity being assessed by measuring the release of hemoglobin. The substrates for phospholipase C are phospholipids, which are major components of the erythrocyte membranes. Hence, the phospholipids in the membranes are viewed as being "immobilized." Perhpas such immobilization of substrate may be a requisite to the inhibition phenomenon.




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Copyright © 1977 by the American Association for Clinical Chemistry.