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Clinical Chemistry 23: 1492-1498, 1977;
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Clinical Chemistry, Vol 23, 1492-1498, Copyright © 1977 by American Association for Clinical Chemistry

Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

RC Leif, RA Thomas, TA Yopp, BD Watson, VR Guarino, DH Hindman, N Lefkove and LM Vallarino

We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light-scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare-earth-based fluorochromes, that emit well-defined, spectrally distinct peaks.


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Copyright © 1977 by the American Association for Clinical Chemistry.