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Clinical Chemistry 24: 135-137, 1978;
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Clinical Chemistry, Vol 24, 135-137, Copyright © 1978 by American Association for Clinical Chemistry

Buffer-exchange column for rapid separation of carcinoembryonic antigen from perchloric acid

DE Haagensen Jr, RL Easterday, CA Stolle and SA Wells Jr

A Sephadex G-50 medium (Pharmacia) buffer-exchange column has been developed for rapidly changing the medium for carcinoembryonic antigen from perchloric acid to acetate buffer. Analytical recovery of the extracted antigen exceeded 95%. Plasma for analysis can be so prepared and samples ready for analysis within an hour. The technique also results in uniform assay volume, thus eliminating this variable from the assay.




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Copyright © 1978 by the American Association for Clinical Chemistry.