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Clinical Chemistry 24: 367-368, 1978;
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Clinical Chemistry, Vol 24, 367-368, Copyright © 1978 by American Association for Clinical Chemistry

Spectrophotometry of theophylline-7-acetic acid and theophylline

JA Owen and K Nakatsu

A spectrophotometric assay procedure is described for individually measuring theophylline-7-acetic acid and theophylline in 2.0 ml of serum. Absorbance is a linear function of concentration over a range of 0.5-40 mg/liter for theophylline-7-acetic acid and 0.5-40 mg/liter for theophylline. No endogenous or exogenous compounds were found that interfere with theophylline-7-acetic acid determinations. As with other spectrophotometric theophylline assays, theobromine and, to a slight extent, phenobarbital interfere with theophylline determinations.




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Copyright © 1978 by the American Association for Clinical Chemistry.