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Clinical Chemistry 24: 663-668, 1978;
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Clinical Chemistry, Vol 24, 663-668, Copyright © 1978 by American Association for Clinical Chemistry

Effect of storage on the apparent concentration of folate in erythrocytes, as measured by competitive protein binding radioassay

E Mortensen

The folate concentration in erythrocytes changes during incubation. Using whole blood samples and washed erythrocytes, I find greatly increasing concentrations of folate in washed samples at 37 degrees C, decreasing concentrations in whole-blood samples. This difference in behavior depends on a thermostable, ultrafiltrable factor in plasma. Exclusion of glucose or addition of fluoride did not affect these results. Decreasing concentrations of folate were found when iodoacetate or acetylphenylhydrazine was present. At 4 degrees C the concentrations of folate were stable for 72 h. I discuss possible explanations for the observed changes and their practical significance.




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Copyright © 1978 by the American Association for Clinical Chemistry.