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Clinical Chemistry 24: 782-787, 1978;
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Clinical Chemistry, Vol 24, 782-787, Copyright © 1978 by American Association for Clinical Chemistry

Two-point determination of plasma ammonia with the centrifugal analyzer

AW Bruce, CM Leiendecker and EF Freier

A simple, convenient, and rapid method for determining ammonia in plasma by the glutamate dehydrogenase reaction is described for the centrifugal analyzer. The measuring principle is fixed-time, with NADH as the coenzyme. ADP is added to stabilize glutamate dehydrogenase and prevent interference from endogenous plasma ADP. The reaction is linear to 400 mumol of ammonia per liter. The plasma sample volume is 100 microliter and the whole procedure takes only 25 min, including the 15- min preincubation. The normal range for venous plasma was 44 +/- 13.5 (SD) mumol of ammonia per liter.




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Copyright © 1978 by the American Association for Clinical Chemistry.