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Clinical Chemistry 24: 1191-1193, 1978;
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Clinical Chemistry, Vol 24, 1191-1193, Copyright © 1978 by American Association for Clinical Chemistry

Improved continuous-flow (SMAC) determination of serum albumin

G Cederblad, BE Hickey, A Hollender and G Akerlund

The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.




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Copyright © 1978 by the American Association for Clinical Chemistry.