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Clinical Chemistry 24: 1595-1598, 1978;
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Clinical Chemistry, Vol 24, 1595-1598, Copyright © 1978 by American Association for Clinical Chemistry

Direct solid-phase 125I radioimmunoassay of serum cortisol

P Brock, EW Eldred, JE Woiszwillo, M Doran and HJ Schoemaker

We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.




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Copyright © 1978 by the American Association for Clinical Chemistry.