Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 25: 1954-1964, 1979;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rautela, G. S.
Right arrow Articles by Miller, W. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rautela, G. S.
Right arrow Articles by Miller, W. K.

Clinical Chemistry, Vol 25, 1954-1964, Copyright © 1979 by American Association for Clinical Chemistry

Response-surface co-optimization of reaction conditions in clinical chemical methods

GS Rautela, RD Snee and WK Miller

We describe the theory and applications of the response-surface approach to simultaneous optimization (co-optimization of multiple interdependent variables. The co-optimization experiments are designed according to a selected response-surface model. Computer-assisted analysis includes fitting the data to the model, testing the resulting fit for statistical validity, and plotting contour maps of the model for simple interpretation. Co-optimizations of the reaction parameters of three methods--creatine kinase, lipase, and aspartate aminotransfrease--are discussed to illustrate the application of the approach. In contrast to the commonly used optimization strategies in which each factor is varied in turn while the others are kept constant, the response-surface approach allows study of several responses (reaction rates, sensitivities) and effects (linear, curvature, interaction) at the same time. It also allows determination of accurate optima, which is necessary for the formulation of analytically reliable clinical methods.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
K. Lorentz
Routine {alpha}-Amylase Assay Using Protected 4-Nitrophenyl-1,4-{alpha}-D-maltoheptaoside and a Novel {alpha}-Glucosidase
Clin. Chem., May 1, 2000; 46(5): 644 - 649.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
H. Thakkar, D. J. Newman, P. Holownia, C. L. Davey, C.-C. Wang, J. Lloyd, A. R. Craig, and C. P. Price
Development and validation of a particle-enhanced turbidimetric inhibition assay for urine albumin on the Dade aca(R) analyzer
Clin. Chem., January 1, 1997; 43(1): 109 - 113.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1979 by the American Association for Clinical Chemistry.