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Clinical Chemistry 25: 2038-2039, 1979;
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Clinical Chemistry, Vol 25, 2038-2039, Copyright © 1979 by American Association for Clinical Chemistry

Is antiglycolysis required for routine glucose analysis?

K Sazama, EA Robertson and RA Chesler

We obtained -68 pairs of simultaneously drawn serum and fluoride- oxalate plasma samples from patients and analyzed them by a continuous- flow (AutoAnalyzer II) glucose oxidase method. Glucose concentrations ranged from 370 to 3530 mg/L. Glucose concentrations for samples obtained in the fluoride-oxalate preservative averaged 42 +/- 35 mg/L (mean +/- SD) higher than serum. The magnitude of this difference was independent of glucose concentration. Linear-regression analysis of 270 pairs for which the time from collection to separation was recorded indicated that the difference between serum and plasma increased by 0.32 mg/L per minute of delay over a time span of 15 to 295 min. These differences are smaller than those described in standard textbooks. We conclude that, with the specimen-handling process used in our hospital, serum glucose determinations are clinically acceptable.




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Copyright © 1979 by the American Association for Clinical Chemistry.