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Clinical Chemistry 25: 2040-2042, 1979;
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Clinical Chemistry, Vol 25, 2040-2042, Copyright © 1979 by American Association for Clinical Chemistry

A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue

RS Boethling and TL Weaver

This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.




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Copyright © 1979 by the American Association for Clinical Chemistry.