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Clinical Chemistry 25: 215-217, 1979;
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Clinical Chemistry, Vol 25, 215-217, Copyright © 1979 by American Association for Clinical Chemistry

New saccharogenic determination of alpha-amylase in serum and urine

L van Leeuwen

I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed before measurement of amylase activity is begun.




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Copyright © 1979 by the American Association for Clinical Chemistry.