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Clinical Chemistry 25: 227-229, 1979;
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Clinical Chemistry, Vol 25, 227-229, Copyright © 1979 by American Association for Clinical Chemistry

Enzyme-linked immunosorbent choriomammotropin assay

D Dittmar, N Monji, A Cid, H Malkus and A Castro

We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assayed 10 times on the same day, was 5.7%. The coefficient of variation for nine samples (3.5 to 9.0 mg/L) assayed on five different days was 7.9%. Forty-eight clinical samples were assayed (y) and compared with results obtained by radial immunodiffusion (x). The resulting regression equation was: y = 1.05x + 0.78; r = 0.91.




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Copyright © 1979 by the American Association for Clinical Chemistry.