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Clinical Chemistry 25: 366-370, 1979;
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Clinical Chemistry, Vol 25, 366-370, Copyright © 1979 by American Association for Clinical Chemistry

Antibodies to native DNA: detection by immunoperoxidase assay and determination of their immunoglobulin classes

AO Vladutiu, DA Palumbo and JE Asirwatham

Antinuclear antibodies are almost always found in sera of patients with systemic lupus erythematosus. To differentiate antinuclear antibodies from antibodies to DNA in the recently described Crithidia luciliae assay, we developed an immunoperoxidase technique for detecting antibodies to native, double-stranded DNA and compared results by it with those by the Farr assay. Smears of cultured Crithidia luciliae were incubated with human sera, peroxidase-labeled anti-human IgG serum, and diaminobenzidine. The peroxidase stain was examined by conventional light microscopy, which facilitated differentiation between the kinetoplast and the nuclear staining. The Crithidia assay appeared to be specific for double-stranded DNA antibodies, seemed to be more sensitive than the Farr assay, and allowed us to determine the immunoglobulin classes of antibodies to native DNA. Some patients with systemic lupus erythematosus had only IgM or IgA antibodies to DNA.




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Copyright © 1979 by the American Association for Clinical Chemistry.