| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 25, 596-604, Copyright © 1979 by American Association for Clinical Chemistry
GR Warnick, MC Cheung and JJ Albers
We compared six precipitation methods for high-density-lipoprotein cholesterol quantitation with an ultracentrifugation method, the accuracy of which was improved by correcting for 4% manipulative loss in the d greater than 1.063 fractions. For purposes of comparison, the apoprotein B-associated cholesterol (average 56 mg/L) measured by immunoassay in the d greater than 1.063 fractions was subtracted. In 65 plasma samples from men, women, and children, a heparin-Mn2+ procedure with Mn2+ at 46 mmol/L produced results slightly higher (+16 mg/L), while results with Mn2+ at 92 mmol/L averaged slightly lower (-8 mg/L) than the comparison ultracentrifuge method. Results that were about 5% low were obtained by the dextran sulfate 500-Mg2+ (-25 mg/L) and phosphotungstate-Mg2+ (-31 mg/L) methods. A heparin-Ca2+ method produced results 10% high (+58 mg/L). Results by a polyethylene glycol- 6000 precipitation method were 12% low (-64 mg/L). Precision was better with the two heparin-Mn2+ and the dextran sulfate 500-Mg2+ procedures, with CVs of 4%, intermediate with phosphotungstate-Mg2+ and polyethylene glycol-6000 (CV 6-7%), and poorest with heparin-Ca2+ (CV 10%). Precipitation by phosphotungate-Mg2+ appeared more sensitive to reagent concentration and temperature variations than either the heparin-Mn2+ or dextran sulfate 500-Mg2+ methods. We conclude that these precipitation methods are not equivalent, but give rise to significant systematic differences in high-density-lipoprotein cholesterol quantitation.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  C. G. Magnussen, O. T. Raitakari, R. Thomson, M. Juonala, D. A. Patel, J. S.A. Viikari, J. Marniemi, S. R. Srinivasan, G. S. Berenson, T. Dwyer, et al. Utility of Currently Recommended Pediatric Dyslipidemia Classifications in Predicting Dyslipidemia in Adulthood: Evidence From the Childhood Determinants of Adult Health (CDAH) Study, Cardiovascular Risk in Young Finns Study, and Bogalusa Heart Study Circulation, January 1, 2008; 117(1): 32 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.C. Sachet, E.F. Borba, E. Bonfa, C.G.C. Vinagre, V.M. Silva, and R.C. Maranhao Chloroquine increases low-density lipoprotein removal from plasma in systemic lupus patients Lupus, April 1, 2007; 16(4): 273 - 278. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Okazaki, S. Usui, M. Ishigami, N. Sakai, T. Nakamura, Y. Matsuzawa, and S. Yamashita Identification of Unique Lipoprotein Subclasses for Visceral Obesity by Component Analysis of Cholesterol Profile in High-Performance Liquid Chromatography Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 578 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rej Clinical Chemistry through Clinical Chemistry: A Journal Timeline Clin. Chem., December 1, 2004; 50(12): 2415 - 2458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ikewaki, W. Cain, F. Thomas, R. Shamburek, L. A. Zech, D. Usher, H. B. Brewer Jr., and D. J. Rader Abnormal in vivo metabolism of apoB-containing lipoproteins in human apoE deficiency J. Lipid Res., July 1, 2004; 45(7): 1302 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Uauy, C. E Mize, and C. Castillo-Duran Fat intake during childhood: metabolic responses and effects on growth Am. J. Clinical Nutrition, November 1, 2000; 72 (5): 1354S - 1360S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cobbaert, P. G.H. Mulder, H. Baadenhuijsen, L. Zwang, C. W. Weykamp, and P. N.M. Demacker Survey of Total Error of Precipitation and Homogeneous HDL-Cholesterol Methods and Simultaneous Evaluation of Lyophilized Saccharose-containing Candidate Reference Materials for HDL-Cholesterol Clin. Chem., March 1, 1999; 45(3): 360 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-L. Arranz-Pena, J. Tasende-Mata, and F. J. Martin-Gil Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol Clin. Chem., December 1, 1998; 44(12): 2499 - 2505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Frémont, M. T. Gozzélino, M. P. Franchi, and A. Linard Dietary Flavonoids Reduce Lipid Peroxidation in Rats Fed Polyunsaturated or Monounsaturated Fat Diets J. Nutr., September 1, 1998; 128(9): 1495 - 1502. [Abstract] [Full Text]
|
|
|
|
|
|
M. Nauck, W. Marz, and H. Wieland New immunoseparation-based homogeneous assay for HDL-cholesterol compared with three homogeneous and two heterogeneous methods for HDL-cholesterol Clin. Chem., July 1, 1998; 44(7): 1443 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M a Simo, I. Castellano, N. Ferre, J. Joven, and J. Camps Evaluation of a homogeneous assay for high-density lipoprotein cholesterol: limitations in patients with cardiovascular, renal, and hepatic disorders Clin. Chem., June 1, 1998; 44(6): 1233 - 1241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cobbaert, L. Zwang, F. Ceriotti, A. Modenese, P. Cremer, W. Herrmann, G. Hoss, J. Jarausch, R. Turk, W. Marz, et al. Reference standardization and triglyceride interference of a new homogeneous HDL-cholesterol assay compared with a former chemical precipitation assay Clin. Chem., April 1, 1998; 44(4): 779 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Neuzil, J. K. Christison, E. Iheanacho, J.-C. Fragonas, V. Zammit, N. H. Hunt, and R. Stocker Radical-induced lipoprotein and plasma lipid oxidation in normal and apolipoprotein E gene knockout (apoE-/-) mice: apoE-/- mouse as a model for testing the role of tocopherol-mediated peroxidation in atherogenesis J. Lipid Res., February 1, 1998; 39(2): 354 - 368. [Abstract] [Full Text]
|
|
|
|
|
|
M. Okazaki, K. Sasamoto, T. Muramatsu, and S. Hosaki Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC Clin. Chem., October 1, 1997; 43(10): 1885 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nauck, W. Marz, J. Jarausch, C. Cobbaert, A. Sagers, D. Bernard, J. Delanghe, G. Honauer, P. Lehmann, E. Oestrich, et al. Multicenter evaluation of a homogeneous assay for HDL-cholesterol without sample pretreatment Clin. Chem., September 1, 1997; 43(9): 1622 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Huang, J.-T. Kao, and K.-S. Tsai Evaluation of two homogeneous methods for measuring high-density lipoprotein cholesterol Clin. Chem., June 1, 1997; 43(6): 1048 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Harris, V. Galpchian, J. Thomas, E. Iannotti, T. Law, and N. Rifai Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate–Mg2+ method Clin. Chem., May 1, 1997; 43(5): 816 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Borba and E. Bonfa Dyslipoproteinemias in systemic lupus erythematosus: influence of disease, activity, and anticardiolipin antibodies Lupus, January 1, 1997; 6(6): 533 - 539. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Senti, J. Pedro-Botet, J. Rubies-Prat, and F. Vidal-Barraquer Secondary Prevention of Coronary Heart Disease in Patients with Extracoronary Atherosclerosis: A Need for Accuracy of Low Density Lipoprotein Determination Angiology, March 1, 1996; 47(3): 241 - 246. [Abstract] [PDF]
|
|
|
|
|
|
G. Broda, C. E. Davis, A. Pajak, O. D. Williams, S. L. Rywik, E. Baczynska, A. R. Folsom, and M. Szklo Poland and United States Collaborative Study on Cardiovascular Epidemiology : A Comparison of HDL Cholesterol and Its Subfractions in PopulationsCovered by the United States Atherosclerosis Risk in Communities Study and thePol-MONICA Project Arterioscler. Thromb. Vasc. Biol., February 1, 1996; 16(2): 339 - 349. [Abstract] [Full Text]
|
|
|
|
|
|
F. Tato, G. L. Vega, and S. M. Grundy Bimodal Distribution of Cholesteryl Ester Transfer Protein Activities in Normotriglyceridemic Men With Low HDL Cholesterol Concentrations Arterioscler. Thromb. Vasc. Biol., April 1, 1995; 15(4): 446 - 451. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ikewaki, L. A. Zech, M. Kindt, H. B. Brewer Jr, and D. J. Rader Apolipoprotein A-II Production Rate Is a Major Factor Regulating the Distribution of Apolipoprotein A-I Among HDL Subclasses LpA-I and LpA-I:A-II in Normolipidemic Humans Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 306 - 312. [Abstract] [Full Text]
|
|
|
|
|
|
F. Tato, G. L. Vega, A. R. Tall, and S. M. Grundy Relation Between Cholesterol Ester Transfer Protein Activities and Lipoprotein Cholesterol in Patients With Hypercholesterolemia and Combined Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., January 1, 1995; 15(1): 112 - 120. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
