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Clinical Chemistry 25: 622-625, 1979;
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Clinical Chemistry, Vol 25, 622-625, Copyright © 1979 by American Association for Clinical Chemistry

Preparation of cytosol for steroid receptor determinations with the Beckman "Airfuge"

PH Heidemann and JL Wittliff

Preparation of cytosols from several estrogen target organs was compared, with use of the Beckman Airfuge and a preparative ultracentrifuge. The specific estrogen-binding capacities of cytosols prepared with these instruments were indistinguishable. Similarly, the sedimentation profiles of estrogen-receptor species as determined by sucrose gradient centrifugation were identical. We conclude that the Airfuge provides a rapid, accurate, and inexpensive means of preparing cytosols for measurements of steroid receptors in the clinical laboratory.




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Copyright © 1979 by the American Association for Clinical Chemistry.