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Clinical Chemistry 25: 682-685, 1979;
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Clinical Chemistry, Vol 25, 682-685, Copyright © 1979 by American Association for Clinical Chemistry

Phosphatidylglycerol in 261 samples of amniotic fluid from normal and diabetic pregnancies, as measured by one-dimensional thin-layer chromatography

MY Tsai and JG Marshall

We describe a one-dimensional thin-layer chromatographic method for separating lecithin, sphingomyelin, phosphatidylglycerol, and other phospholipids. The occurrence of phosphatidylglycerol in relation to the lecithin/sphingomyelin ratio is reported for 261 amniotic fluid samples. This compound does not consistently appear until the ratio exceeds about 3.1, and occurs less often in samples from diabetic patients than in those from normal patients. The respiratory distress syndrome did not occur when phosphatidylglycerol was present in amniotic fluid although the reverse was not necessarily true. Thus the presence of phosphatidylglycerol offers additional assurance of pulmonary maturity.


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 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Friesen and S. M. Innis
Maternal dietary fat alters amniotic fluid and fetal intestinal membrane essential n-6 and n-3 fatty acids in the rat
Am J Physiol Gastrointest Liver Physiol, March 1, 2006; 290(3): G505 - G510.
[Abstract] [Full Text] [PDF]


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Copyright © 1979 by the American Association for Clinical Chemistry.