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Clinical Chemistry 25: 1254-1258, 1979;
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Clinical Chemistry, Vol 25, 1254-1258, Copyright © 1979 by American Association for Clinical Chemistry

Performance assessment of the GammafloTM automated radioimmunoassay system by assaying for digoxin

R Valdes Jr, G Savory, D Bruns, B Renoe, J Savory and MR Wills

We report an evaluation of the GammafloTM automated continuous-flow radioimmunoassay instrument in which we used a digoxin assay to assess system performance. System operation was based on combined continuous- flow and column-chromatographic techniques. No drift or carryover was detectable in 180 within-assay consecutive determinations performed at a rate of 42 determinations per hour (5 h of continuous operation). Within-assay and between-assay precision were less than 6% (coefficient of variation). The automated method correlated well (r = 0.960 and 0.952, respectively) with two established manual digoxin radioimmunoassay procedures. The data suggest this automated system offers a valid alternative to manual radioimmunoassay procedures in terms of overall precision, simplicity of operation, and sample throughout capacity.




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Copyright © 1979 by the American Association for Clinical Chemistry.