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Clinical Chemistry 25: 1267-1273, 1979;
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Clinical Chemistry, Vol 25, 1267-1273, Copyright © 1979 by American Association for Clinical Chemistry

Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH

PC Kao, NS Jiang and PC Carpenter

A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody- bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.


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