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Clinical Chemistry 25: 1285-1289, 1979;
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Clinical Chemistry, Vol 25, 1285-1289, Copyright © 1979 by American Association for Clinical Chemistry

Comparison of acid phosphatase isoenzymes of human seminal fluid, prostate, and leukocytes

WK Lam, LT Yam, HJ Wilbur, E Taft and CY Li

We used ion-exchange column chromatography and electrophoresis on polyacrylamide gel to compare the acid phosphatase isoenzymes of prostate and leukocytes. The major isoenzyme of the prostate is band 2A; only a trace of band 2B was observed. However, the major isoenzymes of leukocytes are band 4 and band 2B, and only a small amount of band 2A was observed. The three isoenzymes isolated from leukocytes or prostate gland react to the antiserum prepared against the aicd phosphatase isoenzyme of seminal fluid. Acid phosphatases of leukocytes other than the three isoenzymes mentioned above did not interact with the antiserum.




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Copyright © 1979 by the American Association for Clinical Chemistry.