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Clinical Chemistry 25: 1314-1317, 1979;
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Clinical Chemistry, Vol 25, 1314-1317, Copyright © 1979 by American Association for Clinical Chemistry

Measurement of Erythrocyte 2,3-diphosphoglycerate with a centrifugal analyzer

TM Hickey, DE Uddin and LA Kiesow

Intra-erythrocytic concentration of 2,3-diphosphoglycerate is a major determinant of the oxygen affinity of hemoglobin. We report here the adaptation of its assay to a centrifugal analyzer, with use of a commercially available reagent. Results are calculated by using the reagent-blank-corrected absorbance change at 340 nm between 120 and 300 s for the samples and a 2.5 mmol/L standard. Under these conditions the standard curve is linear to 5 mmol/L. The compound in a 101-fold aqueous hemolysate is stable for several weeks at either -4 or -70 degrees C. Assay sensitivity and precision are excellent and results agree well with those by the corresponding manual method.




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Copyright © 1979 by the American Association for Clinical Chemistry.