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Clinical Chemistry 25: 1426-1431, 1979;
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Clinical Chemistry, Vol 25, 1426-1431, Copyright © 1979 by American Association for Clinical Chemistry

Solid-phase enzyme immunoassay for serum ferritin

S Anaokar, PJ Garry and JC Standefer

We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6- sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this assay offers a reliable alternative to radioimmunoassay for serum ferritin.




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Copyright © 1979 by the American Association for Clinical Chemistry.