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Clinical Chemistry 25: 1436-1439, 1979;
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Clinical Chemistry, Vol 25, 1436-1439, Copyright © 1979 by American Association for Clinical Chemistry

Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor

PV Sundaram, B Blumenberg and W Hinsch

We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydrogenase solution method. The coefficient of correlation was r = 0.996. A glucose dehydrogenase reactor made starting with 1 mg (250 U) of enzyme was stable during eight weeks of continuous use, that is, for nearly 3500 tests. This reduced the cost of the assay by at least 50-fold, compared with that for a commercial glucose dehydrogenase test pack method.




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Copyright © 1979 by the American Association for Clinical Chemistry.