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Clinical Chemistry 26: 111-114, 1980;
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Clinical Chemistry, Vol 26, 111-114, Copyright © 1980 by American Association for Clinical Chemistry

Simultaneous liquid-chromatographic quantitation of salicylic acid, salicyluric acid, and gentisic acid in urine

BE Cham, F Bochner, DM Imhoff, D Johns and M Rowland

We have developed a specific and sensitive method for the determination of salicylic acid, salicyluric acid, and gentisic acid in urine. Any proteins present are precipitated with methyl cyanide. After centrifugation, an aliquot of the supernate is directly injected into an octadecyl silane reversed-phase chromatographic column, then eluted with a mixture of water, butanol, acetic acid, and sodium sulfate, and quantitated at 313 nm by ultraviolet detection according to peak-height ratios (with internal standard, o-methoxybenzoic acid) or peak heights (no internal standard). The method allows estimates within 25 min. Sensitivity was 0.2 mg/L for gentisic acid, and 0.5 mg/L for both salicyluric and salicylic acid (20-micro L injection volume); response was linear with concentration to at least 2.000 g/L for salicylic acid and metabolites. Analytical recovery of salicylic acid and metabolites from urine is complete. Intra-assay precision (coefficient of variation) is 5.52% at 7.5 mg/L for salicylic acid, 5.01% at 9.33 mg/L for salicyluric acid, and 3.07% at 7.96 mg/L for gentisic acid. Interassay precision is 7.32% at 7.51 mg/L for salicylic acid, 5.52% at 8.58 mg/L for salicyluric acid, and 3.97% at 8.32 mg/L for gentisic acid. We saw no significant interference in urine from patients being treated with various drugs other than aspirin.


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D.K. Patel, A. Hesse, A. Ogunbona, L.J. Notarianni, and P.N. Bennett
Metabolism of Aspirin after Therapeutic and Toxic Doses
Human and Experimental Toxicology, January 1, 1990; 9(3): 131 - 136.
[Abstract] [PDF]

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 Hum Exp ToxicolHome page
D.K. Patel, A. Ogunbona, L.J. Notarianni, and P.N. Bennett
Depletion of Plasma Glycine and Effect of Glycine by Mouth on Salicylate Metabolism During Aspirin Overdose
Human and Experimental Toxicology, January 1, 1990; 9(6): 389 - 395.
[Abstract] [PDF]


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