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Clinical Chemistry 26: 133-137, 1980;
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Clinical Chemistry, Vol 26, 133-137, Copyright © 1980 by American Association for Clinical Chemistry

The multilayered film analyzer: glucose in serum, plasma, cerebrospinal fluid, and urine; and urea nitrogen in serum and plasma

K Warren, NP Kubasik, BB Brody, HE Sine and JP D'Souza

Some basic laboratory performance criteria were studied for the Eastman Kodak glucose and urea nitrogen analyzer. Serum, plasma, urine, and cerebrospinal fluid were tested. Precision, both "within-day" and "between-day," for both analytes was less than 2.2% (CV). Both analytes demonstrated linearity, with about 100% recovery of added substrates. Comparisons with continuous-flow procedures demonstrated good correlation. A variety of sera and plasmas can be used for glucose analysis; oxalate--fluoride-treated plasma is unacceptable for use in analysis for urea nitrogen. We saw no effects on glucose or urea nitrogen analysis from hemolysis, lipemia, icterus, some common drugs, ammonia, or abnormally high protein concentrations. Minimum amounts detectable were: glucose (serum) 104 mg/L; glucose (cerebrospinal fluid) 100 mg/L; and urea nitrogen (serum) 21 mg/L. Calibration procedures are discussed for protein-based and aqueous samples.




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